Desarrollo y aplicación de técnicas innovadoras de biología molecular para mejorar la detección del SARS-CoV-2 durante la pandemia de COVID-19

Development and application of innovative molecular biology techniques to improve the detection of SARS-CoV-2 during the COVID-19 pandemic

Resumen

Molecular biology has been key in the detection and diagnosis of SARS-CoV-2 virus since the beginning of the COVID-19 pandemic. Although RT-PCR is the Gold Standard technique to detect the virus, the socio-political and epidemiological reality of each region of the planet has motivated the use of other complementary techniques for its detection. The use of molecular techniques such as digital PCR, LAMP-PCR, CrispR cas and NGS have allowed rapid and accurate detection of the virus, especially in people with symptoms but with a low viral load. Thus, the aim for the future is to improve their use and availability in the field. In view of their development during the pandemic, it has been seen that these molecular diagnostic techniques have great potential for the diagnosis of other infectious diseases, as they offer advantages such as greater sensitivity and specificity compared to traditional diagnostic methods. Thus, they can significantly improve the accuracy and speed of processing, which can lead to better management and prevention of infectious diseases worldwide.